Each individual reaction was done in a ultimate quantity of twenty five l that contains 1 l cDNA and a pair of x SYBR Eco-friendly PCR Grasp combine. The amplification profile was denaturation at ninety four for 2 min, followed by forty cycles of 94 for ten sec, 58 for 30 sec. On the finish with the PCR cycles, melting curve analysis of your PCR merchandise was performed to validate the amplifi